Integrin beta 2 anticorps

N° du produit ABIN94010

L’anticorps Souris Monoclonal anti-Integrin beta 2 a été validé pour FACS, WB et IP. Il convient pour détecter Integrin beta 2 dans des échantillons de Humain. Il y a 3+ publications disponibles.

Aperçu rapide pour Integrin beta 2 anticorps (ABIN94010)

Antigène: Integrin beta 2 (ITGB2)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Integrin beta 2 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Clone: MEM-148

Détail du produit anti-Integrin beta 2 anticorps

Fonction: Anti-Hu CD18 Purified

Specificité: The antibody MEM-148 recognizes an extracellular epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit, beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

Aucune reactivité croisée: Porc

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Peripheral blood mononuclear cells

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-5 μg/mL. The antibody MEM-148 is an excellent marker of activated myeloid cells.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-Integrin beta 2 (ABIN94010)

Aubert, Yoon, Sloan, Spear, Jerome: "The virological synapse facilitates herpes simplex virus entry into T cells." dans: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, (2009) (PubMed).

Drbal, Angelisová, Cerný, Hilgert, Horejsí: "A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins." dans: Immunobiology, Vol. 203, Issue 4, pp. 687-98, (2001) (PubMed).

Drbal, Angelisová, Cerný, Pavlistová, Cebecauer, Novák, Horejsí: "Human leukocytes contain a large pool of free forms of CD18." dans: Biochemical and biophysical research communications, Vol. 275, Issue 2, pp. 295-9, (2000) (PubMed).

Détails sur Integrin beta 2

Antigène: Integrin beta 2 (ITGB2)

Autre désignation: CD18

Sujet: Integrin subunit beta 2,CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.,Integrin beta2, Complement receptor C3 subunit beta, ITGB2

ID gène: 3689

UniProt: P05107

Pathways: Signalisation NF-kappaB, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation